Electrophoresis ee serum iyo kaadida. Electrophoresis borotiinada serum: qoraalka xeerka

In plasma dhiigga aadanaha waxaa jira go'an qaybaha protein. Waxay kala duwan ee ay ka kooban, qaab-dhismeedka iyo dhaqdhaqaaq deegaanka gaar ah yihiin, qabashada hadda korontada. In dhisida iyo kala protein guud ahaan, taas oo localizes in plasma ee jajabka protein kala duwan this. In qabashada serum ka electrophoresis ogaato xiriirka ka tiro ka mid ah qaybaha protein shaqsi iyo dhismaha. Waxaa lagama maarmaan ah in la ogaado in a ifafaale bahal kala duwan aadanaha, sida caabuqa ama cudurka kansarka. Waa electrophoresis protein ah ee serum waa muhiim in qabashada cudurka cudurada kala duwan.

Nuxurka habka

Wixii jajab protein cholesterol isticmaalo Electrophoresis ee serum, mabda'a ah kaas oo ku salaysan dhaqdhaqaaqa kala duwan ee qaybaha protein duurka ah ee korontada ku. Qaabkan baaritaanka waa badan oo sax ah iyo wargelin ah, ka duwan caadiga ah guud ahaan falanqaynta dhiigga. Laakiin electrophoresis this muujinaysaa oo keliya dhowr ah oo ka mid ah jajabka protein, qaabka iyo xadka ka mid ah geeddi-socodka bahal foom guud. Falanqaynta cilmi u ogolaanaya xirfadlayaasha caafimaad si aad u ogaato waxa uu yahay saamiga jajabyada protein lagu arkay bini'aadanka, iyo si loo ogaado waxyaabaha gaarka ah ee cudurada ismaqalkii cudur gaar ah.

Noocyada jajabka protein

Inta badan dheecaan ugu weyn ee aadanaha dhalay ama dhiig, koobnaan borotiinada. Tirada guud ee iska caadi ah waa gudahood 60-80 g / l. Waayo, falanqaynta sax electrophoresis ee serum warqad. Daraasad Tani waa habka ugu badan ee falanqaynta. dhexdhexaad ugu weyn waa warqad filter gaar ah. Its feature ugu muhiimsan - absorbability sare. waraaqaha noocan oo kale ah uu u nuugo karaa biyo badan culeyska in 130-200 jeer. Iyada oo ku xidhan electrophoresis qalabka loo isticmaalo warqad socdaa 4-16 saacadood. Waxaa la socday unit ee dhismayaasha protein. Markaas, strip warqada lagu daaweeyaa Daac gaar ah falanqaynta. Farsamadan waa kuwa ugu badan ee ka laboratories caafimaad. Sababo la xiriira saamaynta korontada hadda jajab protein, si xun u amray, oo ku dhaqaaqin xagga koronto ku wanaagsan soo oogay. Sababo la xiriira qaybaha protein dhiigga ku kala qaybsan yihiin 5 jajab loo yaqaan:

• albumin;
• Daawynta ₁ globulins;
• Daawynta ₂ globulin;
• β - globulin;
• γ-globulin.

Albumins xun lagu soo eedeeyay, ayaa la yar oo jajab oo kale, miisaanka kelli marka la barbar dhigo. Sababo la xawaaraha dhaqdhaqaaqa this waa ka sii badan inta kale ee xisbiyadii, oo waxay ku yaalaan jahooyinka ka aagga laga bilaabo. Saddexda jajab dhaqaaqo globulin ugu horeysay ee xawaare gaabis ah, sababta oo ah miisaanka. Laakiin xawaaraha ugu yar ayaa laga diiwaan geliyay γ-globulins. borotiinada Kuwanu waa culus oo waaweyn, marka loo eego dhinacyada kale. Waxay u talin waa ku dhawaad dhexdhexaad ah, sidaas jajab protein this aan la wareejiyay line ah.

baahida loo qabo in la isticmaalo

Currently, electrophoresis of falanqayn serum waxaa inta badan sameeyay ogaanshaha sax ah ee cudurka. Falanqaynta ayaa la siin karaa sidii therapist a, si dhakhaatiir koobto profile. Tilmaamayaasha noqon doonaa on Research:

• caabuq kala duwan;
• nooca chronic cudurka;
• geeddi socodka bahal ah ee unugyada connective;
• dhiig bax gudaha ah,
• neoplasms malignant.

Diyaarinta loo gaarsiiyo falanqaynta

In si ay u bartaan dhaqanka natiijada kuwanu waxay ahaayeen kuwo sax ah, ugu yaraan 8 saacadood ka hor deeq dhiig loo baahan yahay ilaa siin cunista soo dhaweynta. Intaa waxaa dheer, waxaa lagama maarmaan ah in ay ku heshiiyaan on qaadashada daawada, haddii ay jirto, iyadoo dhakhtarka xaadirka ah.

Sanbal dhiig ah

Waayo, natiijada ayaa si qalad ah ukas, waxaa lagama maarmaan ah si loo yareeyo suurtagalnimada ee xinjirowga dhiigga ee go'aaminta a jajab protein iyo protein total. Electrophoresis ee serum dhiigga waxaa si taxadar leh ku qaadeen, iyadoo ay jirto waa suurtagalnimada ah dhalanrog natiijada ay sabab u tahay fibrinogen. Waxaa qarin karaan borotiinada aan caadi ahayn ama lagu qaldin iyaga la.

qiimaha caadiga ah

maalmo ka dib markii gudahooda laga billaabo taariikhda saamiga diyaar u yahay inuu baaro electrophoresis borotiinada serum. Norma helay qaybaha waxqabadka ee dadka waaweyn:

1. protein Total - 63-82 g / l.
2. Albumin - 40-60% ka mid ah tirada guud ee jajabka.
3. Daawynta-globulins ₁ - 2-5%.
4. Daawynta-globulins ₂ - 7-13%.
5. β-globulins - 8-15%
6. γ-globulins - 12-22%.

Ma u baahan tahay si ay u falanqeeyaan

Beddelidda xaddiga jajab protein kasta ee kor ama hoos ayaa tilmaamaya laga yaabaa in horumarinta cudurada gaar ah. Si aad u hesho macluumaad lagu kalsoonaan karo oo ku saabsan arrintan u baahan electrophoresis borotiinada serum. Natiijooyinka kelmadaha fududeeyo xirfadlayaasha caafimaadka cudurka iyo doorashada daaweynta.

Kororka albumin

Bilowgii, loo ogaado xaddiga albumin ee falanqaynta natiijada. Kororka jajab this hadli karaa fuuq-bax. Tani waxay dhici kartaa haddii bukaanku ku dhacdaa matag dheer ama cudurada nidaamka dheef shiidka ah. Albumin sidoo kale kordhin dhacdaa gubasho dhul badan oo ah maqaarka.

albumin dhimay

Wax badan ayaa halis, haddii ee jirka hoos u xaddiga albumin, waxaa ka hadli kara oo ka mid ah heley soo socda:

1. Xanuunada ee kelyaha iyo beerka.
2. Pathology of mareenka caloosha iyo mindhicirka.
3. geeddi socodka faafa.
4. Rabshadaha nidaamka wadnaha.
5. Dhiig.
6. Carcinogenic.
7. Sepsis.
8. Ruumatiisimka.

hoos u dhac A yar ee qadarka albumin sidoo kale waxay noqon kartaa:

1. In hooyooyinka uurka leh.
2. In qiyaasood oo xad-dhaaf ah ee daroogada.
3. Inta lagu guda jiro qandho daba dheeraaday.
4. In sigaarka culus.

Beddelidda tirada α1-globulins

Yaraynta xaddiga A1-globulins diiwaangeliyey deficiency ah α ₁ -antitrypsin. Kordhinta dhibic isku mid sii daridda ah caabuq ee jirka, xanuunada ku dhaca beerka ee bololka nudaha.

Yaraynta α2-globulins

waxaa iska diiwaan gelin in diabetes, caabuq ganaca, in dhallaanka la joonis ee hepatitis asal ahaan sun ah. Waxay muujinaysaa aan sax ahayn, cunto dheeli tirnayn.

Kororka α2-globulins

Waxay dhacdaa ka yar shuruudaha soo socda:

1. Inflammations, gaar ahaan iyadoo ay goobjoog ka ahaayeen exudates carow ah (caabuq sambabka iyo talaabooyin kale oo la joogitaanka malax).
2. xanuunada unugyo (tus, cudurka rheumatic).
3. Carcinogenic.
4. Wakhtiyada soo kabashada ka dib markii gubasho.
5. lug kelyaha.

Intaa waxaa dheer, sida dabeecad ifafaale of hemolysis dhiig giisa inta lagu guda jiro daraasadda ah.

Kordhinta β-globulins

Hyperlipidemia lagu muujiyay at (kordhaya xaddiga subaga dhiiga ku jira), beerka iyo kelyaha cudurada. waxaa lagu ogaan karaa iyadoo boog furan caloosha, iyo hypothyroidism (thyroid shaqada disorder). jajab jaban ayaa lagula socdaa gipobetalipoproteinemii (kordhay betalipoprotein dhiig qayb ah).

Isbeddelka ku jajabka ee γ-globulins

kooxaha waxaa ka mid ah in immunoglobulins xubin ay. Sidaa darteed kordhiyo γ-globulins diiwaan at fashilaad in xasaanadda. Tani waxay badanaa ka dhacdaa marka noocyo kala duwan oo caabuqa, horumarinta geedi socodka bararka, badala in unugyada iyo gubi dhaawac. koritaanka γ-globulins ayaa lagu arkay bukaanka qaba hepatitis chronic. Ku dhawaad xaalad la mid ah waa caan cirrhosis. Xaaladaha daran cudurkan xaddiga jajabka γ-globulin protein si weyn u ka badan yahay albumin. cudurrada qaarkood Under laga yaabaa in formation of γ-globulins ku fashilmaan, oo waa horumarinta borotiinada modified ee dhiigga - paraprotein. immunoelectrophoresis - waxbarasho dheeraad ah waxaa loo sameeyaa si loo ogaado nooca horumarinta noocan oo kale ah. hannaankii Tani waa caadiga ah ee cudurka myeloma Waldenstrom iyo cudurada.

In la kordhiyo γ-globulins ayaa sidoo kale soo jireenka ah ee ka heley soo socda:

• lupus erythematosus;
• endotelioma;
• sameeyaan rheumatoid arthritis;
• osteosarcoma;
• leukemia lymphocytic chronic;
• candidiasis.

Yaraynta xaddiga γ-globulins

Hoos u dhaca γ-globulins kala qaybsan yihiin 3 nooc:

1. Biyaha cusbada (caadiga ah ee carruurta saddex ilaa shan bilood).
2. Congenital (koraya dhalashada ka dib).
3. Idiopathic (marka Jidka horumarka aan la xaqiijin karo).

hoos u dhac Secondary diiwaan gashan horumarinta cuduro oo keena yaraadan ee habka difaaca jirka. Recently ku dhaqanka caafimaad sii kordhaysa waa falanqayn si loo ogaado tirada of prealbumin. Caadi ahaan, waxbarasho noocan oo kale ah ku qaadeen bukaanada ku jira daryeelka degdegga ah.

Yaraynta tirada prealbumin waa baaritaan aad u muhiim ah oo sax ah si loo ogaado dhismeedka of la'aanta protein ee jirka ka mid ah bukaanka. Intii lagu guda jiray falanqayn ku sameeyaa-shiid borotiinka iyo hagaajinta prealbumin in bukaanadaan.

electrophoresis kaadida

Mabda'a falanqaynta noocan ah ay la mid tahay fulinta electrophoresis of technology serum. Waxaa la sameeya baaritaanka badan oo sax ah ama ogaanshaha cilladaha kale. Intaa waxaa dheer, falanqaynta noocan ah kaa caawin doontaa in la garto bukaanka joogitaanka proteinuria.

gunaanad

Electrophoresis ee serum iyo kaadida - habab muhiim ah in cudurka cudurada faafa kala duwan. Thanks to habka cilmi-baarista iyo sax, waxay kaa caawin si loo ogaado nooca cudurada. ogaanshaha sax ah - hab la hubo in la saxo daaweynta iyo ka soo kabashada buuxda.